Title
Sex identification of white-lipped peccary (Tayassu pecari) by multiplex PCR with ZFX and ZFY peccary (Tayassu pecari) by multiplex PCR with ZFX and ZFY specific primers and crossamplification in collared peccaries (Pecari tajacu)
Author(s)
Rufo, D.A.;Keuroghlian, A.;Miyaki, C.Y.;Biondo, C.
Published
2015
Publisher
Suiform Soundings
Abstract
Accurate sex identification of freeranging animals is fundamental to understand many aspects of their ecology, including population dynamics, dispersal, social structure and behavior (e.g., Crawford et al., 2008; Lucchini et al., 2002; Woods et al., 1999). Advances in molecular techniques have enabled researchers to address these questions by analyzing DNA extracted from noninvasive samples like feces and hair (Reed et al., 1997, Vigilant, 1999). Noninvasive sampling is especially important for studies of species that are difficult to capture or at risk of extinction (Hedmark et al., 2004). However, this kind of sample usually provides low quality and quantity of DNA, which is associated with an increased risk of DNA contamination and relatively high rates of amplification and genotyping errors (Taberlet et al., 1999). Since the main constraint imposed by DNA degraded samples is the size of the amplified fragment, the ideal is to develop specific primers sets to amplify short fragments (ideally < 300bp, Buchan et al., 2005).
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PUB15700