Title
An alternative minimally invasive technique for genetic sampling of bats- Wing swabs yield species identification
Author(s)
Player, D., Lausen C.L., Zaitlin, B., Harrison, J., Paetkau, D., Harmston, E.
Published
2017
Publisher
Wildlife Society Bulletin 41: 590-596
Abstract
Bat species are traditionally identified morphologically, but in some cases, species can be difficult to differentiate. Wing punches (biopsies) of wing or tail membranes are commonly used to collect tissue for DNA analysis, but less invasive techniques are preferable. As such, DNA acquired using buccal and wing swabs or from fecal pellets are increasingly being employed. This study compared a dry swabbing technique with the wing biopsy technique for DNA collection. They compared species identification between tissue biopsies and wing swabs collected from bats in Alberta and British Columbia, Canada, between April and November, 2014, and September and October 2015. Species identification was achieved with varying
methods of field collection and lab processing. DNA was extracted, sequenced, and compared with reference sequences and field identifications.
Full Citation
Player, D., Lausen, C., Zaitlin, B., Harrison, J., Paetkau, D. and Harmston, E. (2017), An alternative minimally invasive technique for genetic sampling of bats: Wing swabs yield species identification. Wildl. Soc. Bull., 41: 590–596. doi:10.1002/wsb.803
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